Research and Techniques: Molecular Pathology


Liam Chen, MD, PhD

Associate Director, Molecular Diagnostics Laboratory, Johns Hopkins School of Medicine, USA

Short biography

Dr.Chen is the associate director of molecular diagnostics laboratory at Johns Hopkins Hospital and Johns Hopkins School of Medicine.Dr.Chen received his medical residency and fellowship training at Harvard Medical School. He has over 150 scholarly works including poster presentation, review articles, research publications, and book chapters. He also serves as the topic editor for Frontiers Oncology. His main research interest is in the field of molecular oncology and precision medicine.

Publications: Peer-reviewed Articles

  1. Chen L, Lu G, Lu H. cDNA library construction from compacted eight-cell mouse embryos and differential screening for specific-expressed cDNA clones. Hunan Yi KeDaXueXueBao 22 (2), 95-8
  2. Chen L, Reece C, O'Keefe SL, Hawryluk GW, Engstrom MM, Hodgetts RB.  Induction of the early-late Ddc gene during Drosophila metamorphosis by the ecdysone receptor. Mech Dev. 2002. 114(1-2):95-107.
  3. Chen L, O'Keefe SL, Hodgetts RB. Control of Dopa decarboxylase gene expression by the Broad-Complex during metamorphosis in Drosophila. Mech Dev. 2002. 119(2):145-56.
  4. Chen L, Feany MB. Alpha-synuclein phosphorylation controls neurotoxicity and inclusion formation in a Drosophila model of Parkinson disease. Nat Neurosci. 2005. 8(5):657-63.
  5. Davis MM, Yang P, Chen L, O'Keefe SL, Hodgetts RB. The orphan nuclear receptor DHR38 influences transcription of the DOPA decarboxylase gene in epidermal and neural tissues of Drosophila melanogaster. Genome. 2007. 50(11):1049-60.
  6. Nosho K, Shima K, Kure S, Irahara N, Baba Y, Chen L, Kirkner GJ, Fuchs CS, Ogino S. JC virus T-antigen in colorectal cancer is associated with p53 expression and chromosomal instability, independent of CpG island methylator phenotype. Neoplasia. 2009. 11(1):87-95.
  7. Ogino S, Shima K, Baba Y, Nosho K, Irahara N, Kure S, Chen L, Toyoda S, Kirkner GJ, Wang YL, Giovannucci EL, Fuchs CS. Colorectal cancer expression of peroxisome proliferator-activated receptor gamma (PPARG, PPARgamma) is associated with good prognosis. Gastroenterology. 2009. 136(4):1242-50.
  8. Nosho K, Shima K, Irahara N, Kure S, Baba Y, Kirkner GJ, Chen L, Gokhale S, Hazra A, Spiegelman D, Giovannucci EL, Jaenisch R, Fuchs CS, Ogino S. DNMT3B expression might contribute to CpG island methylator phenotype in colorectal cancer. Clin Cancer Res. 2009. 15(11):3663-71.
  9. Ogino S, Nosho K, Irahara N, Kure S, Shima K, Baba Y, Toyoda S, Chen L, Giovannucci EL, Meyerhardt JA, Fuchs CS. A cohort study of cyclin D1 expression and prognosis in 602 colon cancer cases. Clin Cancer Res. 2009. 15(13):4431-8.
  10. Nosho K, Shima K, Irahara N, Kure S, Firestein R, Baba Y, Toyoda S, Chen L, Hazra A, Giovannucci EL, Fuchs CS, Ogino S. SIRT1 histone deacetylase expression is associated with microsatellite instability and CpG island methylator phenotype in colorectal cancer.  Mod Pathol. 2009.  22(7):922-32.
  11. Ogino S, Nosho K, Baba Y, Kure S, Shima K, Irahara N, Toyoda S, Chen L, Kirkner GJ, Wolpin BM, Chan AT, Giovannucci EL, Fuchs CS. A cohort study of STMN1 expression in colorectal cancer: body mass index and prognosis. Am J Gastroenterol. 2009. 104(8):2047-56.
  12. Chen L, Periquet M, Wang X, Negro A, McLean PJ, Hyman BT, Feany MB. Tyrosine and serine phosphorylation of alpha-synuclein have opposing effects on neurotoxicity and soluble oligomer formation. J Clin Invest. 2009. 119(11):3257-65.
  13. Shankar GM, Chen L, Kim AH, Ross GL, Folkerth RD, Friedlander RM. Composite ganglioneuroma-paraganglioma of the filum terminale. J Neurosurg Spine. 2010. 12(6):709-13.
  14. Stapleton CJ, Walcott BP, Kahle KT, Codd PJ, Nahed BV, Chen L, Robison NJ, Delalle I, Goumnerova LC, Jackson EM. Diffuse central neurocytoma with craniospinal dissemination. J ClinNeurosci. 2012 Jan;19(1):163-6. Epub 2011 Nov 15.
  15. Lyons JL, Gireesh ED, Trivedi JB, Bell WR, Cettomai D, Smith BR, Karram S, Chang T, Tochen L, Zhang SX, McCall CM, Pearce DT, Carroll KC, Chen L, Ratchford JN, Harrison DM, Ostrow LW, Stevens RD. FatalExserohilummeningitis and central nervous Ssystem vasculitis after cervical epidural methylprednisolone injection. Ann Intern Med. 2012. 157(11):835-36.
  16. Bell WR, Dalton JB, McCall CM, Karram S, Pearce DT,Memon W, Lee R, Carroll KC, Lyons JL, Gireesh ED, Trivedi JB, Cettomai D, Smith BR, Chang T, Tochen L, RatchfordJN , Harrison DM, Ostrow LW, Stevens RD, Chen L*, Zhang SX* . Iatrogenic Exserohilum infection of the central nervous system: mycological identification and histopathological findings. Mod Pathol. 2012. 26(2):166-70 (* co-corresponding author).
  17. Yuen D, Leu R, Tse J, Wang S, Chen LL, Chen L. Novel characterization of bEnd.3 cells that express lymphatic vessel endothelial hyaluronan receptor-1. Lymphology. 2014.47(2):73-81.
  18. Sun M, Chen L. Studying tauopathies in Drosophila: A fruitful model. Exp Neurol. 2015. 274:52-57.
  19. Sun M, Oulabi I, Chen L. CRISPR/Cas9 genome editing system in Drosophila. Adv Tech Biol Med. 2015. S1:003.
  20. SM Pinto AL, *Chen L, Friedman R, Grant PE, Poduri A, Takeoka M, Prabhu SP. Sturge-Weber Syndrome: Brain Magnetic Resonance Imaging and Neuropathology Findings. Pediatr Neurol. 2015. 58:25-30 (*co-first author).
  21. Asch-Kendrick RJ, Burton E, Chen L, Nauen D. Schwannoma of the jugular foramen. Brain Pathology 2015. 26 (1): 124-125.
  22. Li T, Tabbarah A, Chen L. Characterization of the link between tau expression, amyloid plaque burden, and neuronal loss in Alzheimer disease. J NeuropatholExp Neurol. 2015. 74(6): 624.
  23. Tabbarah AZ, Robert Bell W, Sun M, Gelwan E, Pletnikova O, Hillis AE, Troncoso JC, Lin MT, Chen L. A rapidly progressive dementia case with pathological diagnosis of FTLD-UPS. ActaNeuropathol. 2016. 132 (2): 309-311.
  24. Sun M, Bell W, LaClair KD, Ling JP, Han H, Kageyama Y, Pletnikova O, Troncoso JC, Wong PC, Chen L. Cryptic exon incorporation occurs in Alzheimer's brain lacking TDP-43 inclusion but exhibiting nuclear clearance of TDP-43. ActaNeuropathol. 2017. 133(6): 923-931.
  25. Sun M, Chen L. A novel method to model chronic traumatic encephalopathy in Drosophila. J Vis Exp. 2017. 125.
  26. Donde A, Wong PC, Chen L. Challenges and advances in gene therapy approaches forneurodegenerative disorders. Curr Gene Ther. 2017. 17(3):187-193.
  27. Chen L. What triggers tauopathy in chronic traumatic encephalopathy? Neural Regeneration Research.2018. 13(6): 985-986.
  28. Zhang F, Chen L. A 72-year-old female with an intraventricular mass. Brain Pathol. 2018. 28(4):587-588.
  29. Zhang Z, Nie S. Chen L. Targeting prion-like protein spreading in neurodegenerative diseases. NeuralRegeneration Research. 2018. 13 (11):1875-1878.
  30. Nie SK, Tan Y, Zhang Z, Chen G, Xiong J, Hu D, Ye K, Zhang Y, Cao X, Zhang ZH, Chen L. Bilateral implantation of shear stress modifier in ApoE knockout mouse induces cognitive impairment and tau abnormalities. Frontiers Aging Neuroscience. 2018. 10:303.
  31. Nino DF, Zhou Q, Yamaguchi Y, Chen L, Guajardo A, Kannan S, Sodhi CP, Hackam D. Cognitive impairments induced by necrotizing enterocolitis can be prevented by inhibiting microglial activation in mouse brain. Science Translational Medicine. 2018. 10(471). pii: eaan0237.
  32. Rodriguez F, Brosnan‐Cashman J, Allen S, Vizcaino MA, Giannini C, Camelo‐Piragua S, Webb M, Matsushita M, Wadhwani N, Tabbarah A, Hamideh D, Jiang L, Chen L, Arvanitis LD, Alnajar H, Barber J, Velasco AR, Orr B, Heaphy CM. Alternative lengthening of telomeres, ATRX loss and h3‐k27m mutations in histologically defined pilocytic astrocytoma with anaplasia. Brain Pathology. 2019. 29 (1):126-140.
  33. Pujada E, Chen L, Rodriguez F. Re:  Pathologic and molecular aspects of anaplasia in circumscribed gliomas and glioneuronal tumors. Brain Tumor Pathology. 2019. Epub.
  34. Donde A, Sun MK, Jeong Y, Wen Y, Wong PC, Chen L. Upregulation of ATG7 attenuates motor neuron dysfunction associated with depletion of TDP-43. 2019. Autophagy. Epub.
  35. Donde A, Sun MK, Ling JP, Braunstein KE, Pang B, Wen X, Cheng X, Wong PC, Chen L. Splicing repression is a major function of TDP-43 in motor neurons. Acta Neuropathologica. 2019. Epub.
  36. Morris M, Kwon R, Chen L. Pediatric idiopathic basal ganglia calcification and spherocytosis with chromosome 8p11 deletion. Journal of Neuropathology and Experimental Neurology. Accepted.

Book Chapter

  1. Diagnostic Molecular Pathology in Practice: A Case-Based Approach. Editor: Iris Schrijver. Publisher: Springer; 2011.
  2. Motor Neuron Disease in Adults. Editor: Mark Bromberg. Publisher: Oxford University Press; 2015.
  3. Pre-Clinical and Clinical Methods in Brain Trauma Research, Editor: Amit Srivastava, Springer
  4. Publishing, 2018.
  5. Top 10 Commentaries in Alzheimer's Disease, Editor: Sravanthi, Avid Science, 2019
  6. Top 5 Contributions in Aging, Editor: Venkata, Avid Science, 2019
  7. The Entomological Guide to Drosophila, Editor, Nova Science Publishers, in press.
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