Marie Carene Nancy Picot-Allain*
Department of Health Sciences, University of Mauritius, Mauritius
Received: 11 October 2018
Accepted: 11 December 2018
Version of Record Online: 20 December 2018
Picot-Allain MCN (2018) Aphloia theiformis (Vahl.) Benn.: A Plant with Various Therapeutic Properties. J Soil Plant Biol 2018(1): 16-20.
Correspondence should be addressed to
Marie Carene Nancy Picot-Allain, Mauritius
Copyright © 2018 Marie Carene Nancy Picot-Allain. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and work is properly cited.
Aphloia theiformis (Vahl.) Benn. is an evergreen medicinal plant possessing multiple therapeutic virtues. Traditional use of the plant among folk populations of Madagascar, Comoros Island, Mascarenes Islands, Seychelles Archipelago, and tropical Africa was geared towards the management of dysentery, fever, rheumatism, ulcers, jaundice, gastrointestinal infections, skin infections, cataract, and diabetes mellitus. Multiple lines of evidence from the literature appraise the biological activities of Aphloia theiformis. Recent in vitro studies support the inhibitory action of Aphloia theiformis methanol leaves extract on key enzymes related to diabetes, obesity, hypertension, Alzheimer’s disease, and kidney stone formation. Such findings have led to the identification of mangiferin. Aphloia theiformis was also reported to possess anti-malaria, anti-Chikungunya, and “anti-aging” properties. The present monograph tries to comprehensively establish the ethnopharmacological uses, biological activities, as well as the botanical description of Aphloia theiformis. It is expected that this comprehensive monograph will support further scientific studies in order to validate other traditional use of Aphloia theiformis, hence opening avenues for the development of novel therapeutic entities.
Keywords: Indian Ocean Islands; Mangiferin; Medicinal Plant; Therapeutic Properties
A strong body of evidence generated by ethnobotanical studies praise the use of medicinal plants by cultures across the globe . Indeed, herbal medicinal systems, practices, and knowledge have been transmitted from one generation to the next over the years . It has been postulated that up to 80% of the population of developing countries still rely on traditional medicine for their primary health case, either because of cultural tradition or scarcity of modern treatment facilities . A substantial number of scientific studies attest that there is currently a renewed interest in medicinal plants for the development of novel therapeutic agents geared towards the prevention and treatment of various pathologies.
Aphloia theiformis (Vahl.) Benn. is presently the only known flowering species of the Aphloiaceae family . Ethnobotanical evidences substantiate the use of A. theiformis by folk cultures of Madagascar, Comoros Island, Mascarenes Islands, Seychelles Archipelago, and tropical Africa for the management of several human ailments . The binomial nomenclature includes the epithet ‘Aphloia’ which stems from the Latin word ‘a’ meaning ‘without’ and ‘phloios’ meaning ‘bark/skin/flower’, while ‘theiformis’ comes from ‘thea’ which means ‘tea’ and ‘formis’ stands for ‘form’ [5,6]. A. theiformis is commonly known as ‘Fandamane’ or ‘Bois goyave/gouyave’ in Mauritius, ‘Change écorce/Bois change écorce’ or ‘Gouyavier marron/Gouyave marron’ in Réunion Island, ‘Bois d’anémone’ in Rodrigues, ‘Bwa merl/Bois merle’ in Seychelles, ‘Voafotsy’, ‘Fandramanana’, or ‘Maramanana’ in Madagascar .
In an endeavor to valorise A. theiformis, this paper attempts to present recent findings on the biological properties of A. theiformis and aims at extending knowledge on its botanical description, ethnopharmacological uses, and phytochemical profile to the scientific community.
A. theiformis is an evergreen treelet reaching 10 to 15 m height (Figure 1A). The trunk, measuring 30 cm in diameter, is black to blackish brown, deciduous in patches; the underlying bark is smooth and pale brown. The young branches are reddish and striated. Mature leaves are variable in shape with narrow to broadly elliptic or narrowly oboval to oboval-elliptic blade. The leaf margin is denticulate to glandular-dentate and measure between 3-8 cm long and 1.5-4.5 cm wide (Figure 1B) . Flowers are auxiliary, solitary or in bunch with white to pale yellow sepals measuring between 5-6 mm in diameter (Figure 1C). The fruits are sub-globular to ovoid-pyriform, turn white at maturity and measure 6-8 mm long and 4-6 mm in diameter (Figure 1D) .
Figure 1: A: Aphloia theiformis whole plant, B: Aphloia theiformis leaves, C: Aphloia theiformis flowers, D: Different plant parts of Aphloia theiformis  1: flowering branch, 2: flower head, 3: developing fruit, 4&6: different types of young leaves, 5: mature leaf, 7: branch with male flower, 8: male flower, 9: fruit.
Literature search revealed the extensive use of A. theiformis in traditional medicine. A. theiformis has been used alone or in combination with other medicinal plants for the management of various diseases including dysentery, fever, rheumatism, ulcers, jaundice, gastrointestinal infections, skin infections, cataract, decrease cholesterol level, diabetes, wound healing, as a diuretic, and anti-pyretic [5,9,10]. Table 1 summarises the preparation methods involving the use of A. theiformis for the management of different human ailments.
Table 1: Ethnopharmacological data on Aphloia theiformis
Major Chemical Constituents
Preliminary phytochemical screening of the crude methanol and aqueous extracts prepared from the leaves of A. theiformis indicated the presence of alkaloids, phenols, flavonoids, saponins, and anthraquinones. Tormentic acid ester glucoside, 23-hydroxytormentic acid ester glucoside, and 6β-hydroxytormentic acid ester glucoside were isolated and identified from A. theiformis methanol leaves extract . More recently, a group of authors reported the presence mangiferin (Figure 2), a xanthone glucoside, in A. theiformis methanol leaves extract .
In vitro testing of the crude methanol extract revealed inhibitory action against enzymes related to diabetes (IC50 67.71, 55.20, 19.73 μg ml–1 for α-amylase, α-glucosidase, and glycogen phosphorylase, respectively), obesity (IC50 939.97, 696.22 μg ml–1 for pancreatic lipase and cholesterol esterase, respectively), Alzheimer’s disease (IC50 186.04 μg ml–1 for acetyl cholinesterase), hypertension (IC50 162.17 μg ml–1 for angiotensin converting enzyme), and kidney stone formation (IC50 148.80 μg ml–1 for urease). In silico molecular docking showed that mangiferin, identified in the crude methanol extract of A. theiformis leaves, docked to the afore-mentioned enzymes, showing π-π interactions and hydrogen bonds mostly. The anti-diabetic properties of A. theiformis, documented from traditional use, can thus be attributed to the presence of mangiferin. Additionally, A. theiformis expressed antiglycation properties and retarded glucose diffusion in vitro. Besides, A. theiformis methanol leaves extract significantly (p<0.05) scavenged nitric oxide (IC50 37.05 μg ml–1), 2, 2-diphenyl-2-picrylhydrazyl (IC50 64.42 μg ml–1), and hypochlorous acid (IC50 41.24 μg ml–1) radicals compared to ascorbic acid (IC50 77.65, 189.91 and 59.18 μg ml–1 for respective assays) .
The immunomodulatory and anti-inflammatory activities of A. theiformis collected in Comoros Island has been investigated. The authors reported that the phenolic rich fraction of A. theiformis exhibited immunosuppressive effect on lymphocyte, immunostimulant effect on monocytes and granulocytes at high doses, and anti-inflammatory-like effect in vivo . The methanol bark extract reduced nitrite release from macrophages with an IC50 value of 19 μg ml–1 showing promising anti-inflammatory effects .
The solvent-free extract of A. theiformis aerial parts obtained from microwave extraction showed potent antiviral effect against ZIKV strains of Africa and Asia and clinical isolates of dengue virus. The extract acted directly on viral particles, preventing attachment to cell surface and entry in host cells . Recently, a group of researchers indicated that A. theiformis displayed high anti-Chikungunya activity, justifying its use during the Chikungunya outbreaks in Reunion Island in 2006 . A. theiformis methanol bark extract (IC50 13.3 μg ml–1) showed antiplasmodial activity against 3D7 chloroquine sensitive strain of Plasmodium falciparum, the most noxious Plasmodium species responsible for malaria in man . The anti-microbial activity of A. theiformis methanol leaves extract against Staphylococcus aureus, Salmonella enteritis, Pseudomonas aeruginosa, Enterobacter cloacae, Bacillus subtilis, Sclerotinia sclerotium, and Candida albicans using the disc diffusion technique has also been reported . The “anti-aging” and photo-protective properties of A. theiformis leaves of Madagascar have been attributed to the presence of xanthones, such as, mangiferin . Mangiferin was reported to inhibit the activity of elastase (IC50 139.64 μM) and collagenase (IC50 253.57 μM), two enzymes targeted for the development of anti-aging agents . As a consequence, pure extract of mangiferin (85-93% w/w) obtained from A. theiformis leaves of Madagascar has been developed for the cosmetic industry .
Cytotoxicity studies on human embryonic kidney cell line HEK-293 using the iCELLigence system have showed that the crude methanol extract and active fractions (IC50 values > 20 μg ml–1) of the leaves of A. theiformis exhibited negligible cytotoxic activity . Besides, A. theiformis methanolic bark extract exhibited moderate cytotoxic activity against WI38 (IC50 58.3 μg ml–1), WS1 (IC50 61 μg ml–1) fibroblasts, and A-549 epithelial cells (IC50 40 μg ml–1) [20,21].
In an attempt to validate ethnopharmacological data, scientific evaluation is essential. Thus, this paper appraised the therapeutic potential of A. theiformis by providing an account of different studies undertaken to evaluate its biological activity. A. theiformis has proved to be a promising plant possessing potent biological activities such as anti-microbial, antioxidant, anti-diabetic, and immunomodulatory properties. Additionally, findings summarised here, highlight the therapeutic function of mangiferin, a phytochemical ubiquitously present in A. theiformis leaves, and substantiate the need for further focus on this lead molecule for the development of novel drugs. However, to further appraise the traditional use of A. theiformis further scientific in vivo evaluations are crucial.
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